未分级区域

一些实验的目的是量化暂停因素的分布,如脊髓灰质炎,整个基因或转录体。脊髓灰质炎和许多其他因素,在转录的开始/结束附近显示暂停(即信号的积累)。由于通常执行缩放以使所有区域具有相同的长度,暂停区域的宽度可以在每个转录中进行不同的缩放。这反过来又会在聚类或其他分析过程中造成偏差。在这种情况下, --unscaled5prime and --unscaled3prime options in computeMatrix can be used. These will prevent regions at one or both end of transcripts (or other regions) to not be excluded from scaling, thereby allowing raw signal profiles to be compared across transcripts. An example of this from Ferrari et al. 2013 如下所示:

../../_images/feature-unscaled0.png

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